OHSS大鼠下丘脑轴内分泌调控机制转录组分析
摘要
摘要：【目的】为了探究OHSS（卵巢过度刺激综合征）对大鼠下丘脑调节甲状腺、肾上腺、垂体轴的影响机制。【方法】试验选用54只6周龄的SD大鼠，随机分为三组后注射不同剂量的孕马血清（PMSG）和人绒毛膜促性腺激素（hCG）进行OHSS模型的建立，获取三组大鼠的下丘脑样本，进行转录组测序，之后对三组中DEGs进行筛选，利用GO功能注释和KEGG富集分析对DEGs进行注释，探索OHSS大鼠下丘脑对甲状腺、肾上腺、垂体调控的分子机制。【结果】M组与D组之间共筛选出216个DEGs，其中上调基因157个，下调基因59个；O组与D组之间共筛选出来237个DEGs，其中上调基因63个，下调基因174个；O组与M组之间共筛选出来490个DEGs，其中上调基因111个，下调基因379个。GO富集分析发现DEGs在多细胞生物过程、多细胞生物发育、细胞分化和细胞发育过程等富集较多；KEGG分析发现DEGs在P13K-Akt、GnRH和Ras等信号通路中显著富集。【结论】 OHSS通过PI3K-Akt、GnRH及Ras等多条信号通路的协同异常引发多器官功能障碍。
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参考
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