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miR-548e-3p 在结直肠癌中的表达及意义

台 维龙, 曹 宇生, 梁 浩源, 黄 许森

摘要

目的:利用生物信息学研究miR-548E-3p的差异,并利用实验验证miRNA在结直肠癌中的作用。方法:利用癌症基因组图谱数据库(The Cancer Genome Atlas,TCGA)下载结直肠癌的miRNA数据,并利用 limma R包筛选mir-548e-3p在结直肠癌中的表达情况。通过转染miR-548e-3p抑制物48小时后提取蛋白样本,利用western blot实验检测抑制物组和对照组中E钙黏蛋白以及N钙粘蛋白的水平变化;通过转染6小时后将SW620以及HCT116细胞消化转移至划痕小室中,待继续转染24小时后观察细胞之间的距离变化。本研究还检测7例结直肠癌患者的癌和远端正常组织之间miR-548e-3p的表达。。结果:mir-548e-3p在TCGA结直肠癌中高表达。在抑制物组检测E钙黏蛋白的表达水平高于正常对照组,且N钙粘蛋白表达水平低于正常对照组。抑制物组的细胞迁移速度明显低于正常对照组。患者癌组织的miR-548e-3p的表达水平明显高于正常肠道组织。结论:mir-548e-3p在结直肠癌中发挥致癌作用,其检测有利于判断结直肠癌的迁移和侵袭能力。

关键词

miRNA;结直肠癌;E钙粘蛋白;N钙粘蛋白

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