紫草素通过 Wnt 信号通路促进人脐静脉内皮细胞血管生成
摘要
信号通路参与糖尿病溃疡愈合过程中血管生成的机制。方法 在高糖处理条件下，用低、中、高（5、10、20umol/L）剂量
紫草素孵育 HUVECs，采用 CCK8 法检测细胞活力，TUNELl 实验观察细胞的凋亡水平。ELISA 检测血管生成关键因子
VEGF、VEGFR2、eNOS的变化，Western Blot 检测血管生成关键因子及Wnt信号通路中相关蛋白的表达。结果 在高糖条件下，
高剂量紫草素处理显著提升了细胞增殖水平（P<0.01）；同时 TUNEL 实验显示紫草素处理明显降低了细胞的凋亡水平。
紫草素处理也显著提升了 VEGF、VEGFR2、eNOS 的表达水平（P<0.05）。通过 WB 实验，发现紫草素处理显著抑制了
GSK-3β 的表达（P<0.05）。给 HUVECs 施以 Wnt 抑制剂，则得到和紫草素处理相反的结果。结论 紫草素通过 GSK-3β
介导的 Wnt/β-Catenin 信号通路的激活，刺激人脐静脉内皮细胞（HUVECs）的增殖，进而促进内皮细胞血管生成。该研
究为深入理解紫草素促进糖尿病溃疡伤口愈合的作用机制提供了一定的理论基础。
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