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建立b型流感嗜血杆菌结合疫苗原液的鉴别方法

董洁, 王立萍, 秦登科, 张勇朝, 潘若文*

摘要

目的：建立b型流感嗜血杆菌结合疫苗原液中破伤风类毒素蛋白的鉴别方法，并进行验证。方法：通过裂解剂预处理供试品后，采用双向免疫扩散法对b型流感嗜血杆菌结合疫苗原液进行鉴别。结果：该方法能呈现显著的免疫沉淀条带。结论：本文建立的方法可有效准确鉴别b型流感嗜血杆菌结合疫苗原液中破伤风类毒素蛋白，同时为其他多糖-蛋白结合疫苗原液及成品的鉴别检查提供新的思路和参考。

关键词

b型流感嗜血杆菌（Hib）；结合疫苗；鉴别；裂解剂；双向免疫扩散法

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参考

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