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Vpr蛋白凋亡在HIV-1中的数据挖掘分析

王丽娜*, 方磊, 郦晶, 蔡莉莉

摘要

背景：凋亡调控是HIV-1感染的机制，Vpr蛋白在凋亡调控起着重要作用机制不明。方法：从GEO数据库中获取3例vpr干预的THP1巨噬细胞与正常细胞数据。筛选差异基因（| log2FC |≥1，FDR<0.05），通过DAVID、KEGG及STRING分析功能富集、蛋白互作及共同表达网络，构建Vpr相关调控网络。结果：筛选到363个差异基因（上调174，下调189），富集于IL-17、TNF信号通路及凋亡等过程。核心基因NFKB1、CXCL2、JUN、FAS等参与Vpr-mRNA-凋亡通路调控网络，如Vpr-NFKB1-IL-17信号通路。结论：本研究揭示Vpr干预下的关键调控基因及通路，为HIV防治提供新靶点。

关键词

HIV；Vpr；数据挖掘；共表达网络

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参考

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