大叶茜草素对宫颈癌细胞增殖、粘附及糖酵解水平的分子机 制研究
摘要
（PI3K/AKT）的调控作用。方法：体外培养人宫颈癌 HeLa 细胞，激活剂组（50 μmol/L 大叶茜草素 +10 μmol/LPI3K/
AKT 通路激活剂 SC-79）和抑制剂组（50 μmol/L 大叶茜草素＋ 2 μmol/L PI3K/AKT 通路抑制剂 LY294002），干预 24 h。
采 用 5- 乙炔基 -2’脱氧尿嘧啶核苷（EdU）法、细胞粘附实验、乳酸、葡萄糖检测试剂盒、蛋白免疫印迹（WB）法进
行分 析。结果：实验组和阳性药物组细胞增殖率、粘附数、乳酸含量、葡萄糖消耗水平、己糖激酶 2（HK2）、乳酸脱氢
酶 A（LDHA）、甘油醛 -3- 磷酸脱氢酶（GAPDH）、p-PI3K、p-AKT（P<0.05）。结论：大叶茜草素作用机制可能与
抑制 PI3K/AKT 通路转导有关。
关键词
全文:
PDF参考
[1]Liu Y, Li L, Li Y. Research progress on tumor-associated
macrophages and inflammation in cervical cancer[J]. Biomed Res
Int, 2020, 20(2): 68-76.
[2]Bray F, Ferlay J, Soerjomataram I, et al. Global cancer
statistics 2018: GLOBOCAN estimates of incidence and mortality
worldwide for 36 cancers in 185 countries[J]. CA Cancer J Clin,
2018, 68(6): 394-424.
[3] 胡鑫洁 , 陈梅 , 李楠 , 等 . 宫颈癌中医分期辨治研
究进展 [J]. 中医药导报 , 2021, 27(3): 155-158+163.
[4] 龚婷 , 张敏 , 王海珠 , 等 . 大叶茜草精油挥发性物
质抑菌及抗氧化活性研究 [J]. 西南师范大学学报 ( 自然科学
版 ), 2019,44(6): 54-59.
[5]Zhe W, Ming L, Mi C, et al. Mollugin has an anti-cancer
therapeutic effect by inhibiting TNF-α-induced NF-κB
activation[J]. INT J MOL SCI, 2017, 18(8): 1619-1631.
[6]International BR. Retracted: Involvement of Nrf2-
mediated upregulation of heme oxygenase-1 in mollugin_xfffe_induced growth inhibition and apoptosis in human oral cancer
cells[J]. Biomed Res Int, 2020, 2020: 1915927.
[7]Wang Z, Li MY, Mi C, et al. Mollugin has an anti-cancer
therapeutic effect by inhibiting TNF-α-induced NF-κB
activation[J]. Int J Mol Sci, 2017, 18(8): 1619.
[8]Bahrami A, Hasanzadeh M, Hassanian SM, et al. The
potential value of the PI3K/AKT/mTOR signaling pathway
for assessing prognosis in cervical cancer and as a target for
therapy[J]. J Cell Biochem, 2017, 118(2): 4163-4169.
[9]Lee CY, Hsin MC, Chen PN, et al. Arctiin inhibits cervical
cancer cell migration and invasion through suppression of s100a4
expression via PI3K/AKT pathway[J]. Pharmaceutics, 2022, 14(2):
365.
[10] 杨美清 , 田建辉 . 中医药防治宫颈癌的研究进展 [J].
中医药导报 , 2019, 25(21): 87-89.
[11]CJiang N, DaiQ, SuX, et al. Role of PI3K/AKT pathway
in cancer: the framework of malignant behavior[J]. Mol Biol Rep,
2020, 47(6): 4587-4629.
[12]Chen YH, Yang SF, Yang CK, et al. Metformin induces
apoptosis and inhibits migration by activating the AMPK/p53 axis
and suppressing PI3K/AKT signaling in human cervical cancer
cells[J]. Mol Med Rep, 2021, 23(1):88.
(21 摘要 Views, 11 PDF Downloads)
Refbacks
- 当前没有refback。