开放期刊系统

梅毒螺旋体外膜蛋白功能及应用的研究进展

陈 建宇, 刁 陈薇, 王 欢*

摘要

梅毒是一种由梅毒螺旋体 (Treponema pallidum, TP) 感染引起的慢性多系统的性传播疾病,近年来梅毒再次成为全
球严重的公共卫生问题。梅毒螺旋体外膜缺乏内毒素,也不产生明显的毒力因子,其外膜蛋白在维持病原性和调节宿主免
疫反应中起着关键作用,有潜在的免疫诊断价值和较强疫苗研究作用。本文对已发现的重要梅毒螺旋体外膜蛋白的结构、
生物学功能,特别是致病机制和潜在诊断价值方面的研究进行了概述,为梅毒防治以及公共卫生领域取得更显著的突破提
供新的思路。

关键词

梅毒螺旋体;外膜蛋白;致病机制;疫苗;研究进展

全文:

PDF

参考

[1]Rosanna W Peeling, David Mabey, Mary L Kamb, et al.

Syphilis[J]. Nat Rev Dis Primers. 2017,3:17073.

[2]Jinlin Chen, Jielite Huang, Zhuoran Liu,et al. Treponema

pallidum outer membrane proteins: current status and

prospects[J]. Pathog Dis. 2022, 80(1):ftac023.

[3]Justin D Radolf, Sanjiv Kumar. The Treponema pallidum

Outer Membrane[J]. Curr Top Microbiol Immunol. 2018,415:1-38.

[4]Xi Luo, Xiaohong Zhang, Lin Gan, et al. The outer

membrane protein Tp92 of Treponema pallidum induces human

mononuclear cell death and IL-8 secretion[J]. 2018,22(12):6039-

6054.

[5]Xie Y, Xu M, Wang C, et al. Diagnostic value of

recombinant Tp0821 protein in serodiagnosis for syphilis[J]. Lett

Appl Microbiol. 2016,62(4):336-343.

[6]Ning Wu, Ni Li, Liping Hu, et al. Immunogenicity and

immunoreactivity of Tp0821 recombinant protein from Treponema

pallidum[J]. Mol Med Rep. 2017,16(1):851-856.

[7] 阳建 , 杨荣志 , 唐诚 . 梅毒螺旋体四种不同膜蛋白在

血清学诊断中的应用研究 [J]. 临床和实验医学杂志 , 2015,

14(06): 457-460.

[8]Kamfai Chan, Thayer Nasereddin, Laura Alter, et al.

Treponema pallidum Lipoprotein TP0435 Expressed in Borrelia

burgdorferi Produces Multiple Surface/Periplasmic Isoforms and

mediates Adherence[J]. Sci Rep. 2016, 10:6:25593.

[9]Karolin Pollok, Ronja Mothes, Carolin Ulbricht, et al.The

chronically inflamed central nervous system provides niches

for long-lived plasma cells. Acta Neuropathol Commun[J].

2017,5(1):88.

[10]Shun Xiong, Zhaoping Liu, Xiaohong Zhang, et al.

Resurgence of syphilis: focusing on emerging clinical strategies

and preclinical models[J]. J Transl Med. 2023,21(1):917.

[11]Chad A Brautigam, Ranjit K Deka, Wei Z Liu, et

al.Insights into the potential function and membrane organization

of the TP0435 (Tp17) lipoprotein from Treponema pallidum

derived from structural and biophysical analyses[J]. Protein Sci.

2015,24(1):11-9.

[12]Lorenzo Giacani, Stephanie L Brandt, Wujian Ke, et

al.Transcription of TP0126, Treponema pallidum putative OmpW

homolog, is regulated by the length of a homopolymeric guanosine

repeat[J]. Infect Immun. 2015,83(6):2275-2289.

[13]Austin M Haynes, Charmie Godornes, Wujian Ke, et al.

Evaluation of the Protective Ability of the Treponema pallidum

subsp. pallidum Tp0126 OmpW Homolog in the Rabbit Model of

Syphilis[J]. Infect Immun. 2019,87(8):e00323-19.

[14]Rui-Li Zhang, Qian-Qiu Wang, Li-Jia Yang. Chemerin

induced by Treponema pallidum predicted membrane protein

Tp0965 mediates the activation of endothelial cell via MAPK

signaling pathway[J]. J Cell Biochem. 2019,20(12):19621-19634.

[15]Matthew McKevitt, Mary Beth Brinkman, Melanie

McLoughlin, et al.Genome scale identification of Treponema

pallidum antigens[J]. Infect Immun. 2005,73(7):4445-4450.

[16]Rui-Li Zhang, Jing-Ping Zhang, Qian-Qiu Wang.

Recombinant Treponema pallidum protein Tp0965 activates

endothelial cells and increases the permeability of endothelial cell

monolayer[J]. PLoS One. 2014,9(12):e115134.

[17]A V Runina, G L Katunin, M A Filippova, et al.

Immunochip for Syphilis Serodiagnostics with the Use of Extended

Array of Treponema pallidum Recombinant Antigens.Bull Exp

Biol Med[J]. 2018,165(6):767-771.

[18]Mary Beth Brinkman, Matthew McKevitt, Melanie

McLoughlin, et al. Reactivity of antibodies from syphilis patients to

a protein array representing the Treponema pallidum proteome[J].

J Clin Microbiol[J]. 2006,44(3):888-891.

[19]Man Xu, Yafeng Xie, Chuanhao Jiang, et al. A novel

ELISA using a recombinant outer membrane protein, rTp0663, as

the antigen for serological diagnosis of syphilis[J]. Int J Infect Dis.

2016, 43:51-57.

[20]Li Zhang, Meixia Deng, Xiaohong Zhang, et al.

Serological evaluation of antigen Tp0693 for diagnosis of

syphilis[J]. Exp Ther Med. 2017,14(5):4729-4736.

[21]Philipp P Bosshard. Usefulness of IgM-specific

enzyme immunoassays for serodiagnosis of syphilis: comparative

evaluation of three different assays. Comparative Study[J]. J Infect.

2013,67(1):35-42.

[22]Vitomir Djokic, Lorenzo Giacani, Nikhat Parveen.

Analysis of host cell binding specificity mediated by the Tp0136

adhesin of the syphilis agent Treponema pallidum subsp. Pallidum.

PLoS Negl Trop Dis[J]. 2019,13(5):e0007401.

[23]Caroline E Cameron, Elizabeth L Brown, Janelle M

Y Kuroiwa, et al. Treponema pallidum fibronectin-binding

proteins[J]. J Bacteriol. 2004,186(20):7019-7022.

[24]Wujian Ke, Barbara J Molini, Sheila A Lukehart, et

al.Treponema pallidum subsp. pallidum TP0136 protein is

heterogeneous among isolates and binds cellular and plasma

fibronectin via its NH2-terminal end[J]. PLoS Negl Trop Dis.

2015,9(3):e0003662.

[25]Mary Beth Brinkman , Melanie A McGill, Jonas

Pettersson, et al. A novel Treponema pallidum antigen, TP0136, is

an outer membrane protein that binds human fibronectin[J]. Infect

Immun. 2008,76(5):1848-1857.

[26]Caroline E Cameron. Syphilis Vaccine Development:

Requirements, Challenges, and Opportunities[J]. Sex Transm Dis.

2018,45(9S Suppl 1):S17-S19.

[27]Simon Houston, Rebecca Hof, Teresa Francescutti, et

al.Bifunctional role of the Treponema pallidum extracellular matrix

binding adhesin Tp0751[J]. Infect Immun. 2011,9(3):1386-1398.

[28]Simon Houston, Rebecca Hof, Lisa Honeyman, et al.

Activation and proteolytic activity of the Treponema pallidum

metalloprotease, pallilysin[J]. PLoS Pathog. 2012;8(7):e1002822.

[29]Michelle L Parker, Simon Houston, Helena Pětrošová,

et al. The Structure of Treponema pallidum Tp0751 (Pallilysin)

Reveals a Non-canonical Lipocalin Fold That Mediates Adhesion

to Extracellular Matrix Components and Interactions with Host

Cells[J]. PLoS Pathog. 2016,12(9):e1005919.

[30]Karen V Lithgow, Rebecca Hof, Charmaine Wetherell,

et al. A defined syphilis vaccine candidate inhibits dissemination

of Treponema pallidum subspecies pallidum[J]. Nat Commun.

2017,8:14273.

[31]Nikhat Parveen, Mark C Fernandez, Austin M

Haynes, et al.Non-pathogenic Borrelia burgdorferi expressing

Treponema pallidum TprK and Tp0435 antigens as a novel

approach to evaluate syphilis vaccine candidates[J]. Vaccine.

2019,37(13):1807-1818.

[32]Dan Liu, Man-Li Tong, Xi Luo, et al. Profile of the

tprK gene in primary syphilis patients based on next-generation

sequencing[J].PLoS Negl Trop Dis. 2019,13(2):e0006855.

[33]Rebecca E LaFond, Barbara J Molini, Wesley C

Van Voorhis, et al. Antigenic variation of TprK V regions

abrogates specific antibody binding in syphilis[J]. Infect Immun.

2006,74(11):6244-6251.

[34]Tara B Reid, Barbara J Molini, Mark C Fernandez, et al.

Antigenic variation of TprK facilitates development of secondary

syphilis[J]. Infect Immun. 2014,82(12):4959-4967.


(2 摘要 Views, 0 PDF Downloads)

Refbacks

  • 当前没有refback。