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探究 Neil2 基因突变对宫颈癌细胞增殖影响及其机制讨论

李 妍, 王 鹤

摘要

目的:研究Neil2基因突变对宫颈癌细胞增殖的影响与其机制。方法:构建表达NEIL2基因的野生型和突变型载体,构建Neil2基因空载型、野生型和突变型SiHa细胞株。CCK8实验检测不同基因型Siha细胞的增殖能力,绘制增殖曲线。结果:同对照小组对比,基因突变类-mut组增速最快,显著超过其余组;Neil2基因野生类-wt组增速放缓,差异有统计学意义(P<0.05)。结论:Neil2基因突变可促进宫颈癌细胞的增殖。

关键词

NeiL2;基因突变;宫颈癌细胞增殖;cck8;机制分析

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参考

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